Journal: Cell Death & Disease

Article Title: Sphingolipid de novo biosynthesis is essential for intestine cell survival and barrier function

doi: 10.1038/s41419-017-0214-1

Figure Lengend Snippet: a Images depicting Sptlc2 KO mouse colon length and quantification at day 6 after tamoxifen treatment. b H&E staining of the colon. Red arrows indicate the top part of crypts. c Goblet cells were stained with periodic acid-Schiff. d Goblet cells were stained with Alcian Blue. e Image of H&E-stained Sptlc2-deficient colon at higher magnification. Black arrows indicate bacterial clusters at the top and in the middle of the mucosa). f TUNEL staining of mouse colon. g Immunostaining for cleaved caspase-3 in the colon. h Western-blot fluorogram and quantification of cleaved caspase-3 in the colon. I-Sptlc2 KO, intestine specific Sptlc2 KO mice. Values represent the mean ± SD, n = 5, * P < 0.01

Article Snippet: Intestinal tissues fixed in 4% formalin were sent to Histowiz Inc. (SUNY Biotechnology Incubator) for immunohistochemical analysis of cleaved caspase-3 and for TUNEL staining.

Techniques: Staining, TUNEL Assay, Immunostaining, Western Blot

Journal: Cell Death & Disease

Article Title: Sphingolipid de novo biosynthesis is essential for intestine cell survival and barrier function

doi: 10.1038/s41419-017-0214-1

Figure Lengend Snippet: a Immunostaining for Ki67. b Quantification of Ki67 in crypts. c DAPI staining to indicate nuclei. d Western-blot fluorogram and quantification of carbonic anhydrase in the colon. Values represent the mean ± SD, n = 5, * P < 0.01. e – f Electron microscopic image of mouse colon. All images are representative of five fields each. CON control, KO intestine-specific Sptlc2 knockout

Article Snippet: Intestinal tissues fixed in 4% formalin were sent to Histowiz Inc. (SUNY Biotechnology Incubator) for immunohistochemical analysis of cleaved caspase-3 and for TUNEL staining.

Techniques: Immunostaining, Staining, Western Blot, Control, Knock-Out

Journal: Cell Death & Disease

Article Title: Sphingolipid de novo biosynthesis is essential for intestine cell survival and barrier function

doi: 10.1038/s41419-017-0214-1

Figure Lengend Snippet: a Immunostaining for E-cadherin in the colon. Black arrows indicate the top part of crypts. b Western-blot fluorogram and quantification of E-cadherin in the colon. c Western-blot fluorogram and quantification of mucin2. d LPS level in mouse plasma. e Western-blot fluorogram and quantification of phospho-ERK and total ERK, phospho-JNK and total JNK, and phospho-p38 and total p38 in the colon of Sptlc2-deficient and control mice. f Plasma IL-6 and TNF-α measurement. Values represent the mean ± SD, n = 5, * P < 0.01. CON control, KO intestine-specific Sptlc2 knockout

Article Snippet: Intestinal tissues fixed in 4% formalin were sent to Histowiz Inc. (SUNY Biotechnology Incubator) for immunohistochemical analysis of cleaved caspase-3 and for TUNEL staining.

Techniques: Immunostaining, Western Blot, Clinical Proteomics, Control, Knock-Out

Journal: Cell Death & Disease

Article Title: Sphingolipid de novo biosynthesis is essential for intestine cell survival and barrier function

doi: 10.1038/s41419-017-0214-1

Figure Lengend Snippet: a H&E staining of Paneth cells and quantification in jejunum. White arrow indicates Paneth cells. b H&E staining of and goblet cells and quantification in jejunum. Yellow arrow indicates goblet cells. Values represent the mean ± SD, n = 30, * P < 0.001. c Western-blot fluorogram and quantification of NPC1L1, CD36, ABCA1, and ABCG8 in the plasma membrane of primary enterocytes. Values represent the mean ± SD, n = 5, * P < 0.01. d Immunostaining for NPC1L1 in the jejunum. e TUNEL and immunostaining for cleaved caspase-3 in the colon. Red arrows indicate apoptotic cells. All images are representative of five fields each

Article Snippet: Intestinal tissues fixed in 4% formalin were sent to Histowiz Inc. (SUNY Biotechnology Incubator) for immunohistochemical analysis of cleaved caspase-3 and for TUNEL staining.

Techniques: Staining, Western Blot, Clinical Proteomics, Membrane, Immunostaining, TUNEL Assay